Convert a Seurat object to an .h5ad file

Convert a Seurat object to an .h5ad file

Usage

srt_to_h5ad(
  srt,
  path,
  features = NULL,
  assay_x = "RNA",
  layer_x = "counts",
  assay_y = c("spliced", "unspliced"),
  layer_y = "counts",
  reductions = NULL,
  graphs = NULL,
  neighbors = NULL,
  convert_tools = FALSE,
  convert_misc = FALSE,
  overwrite = FALSE,
  verbose = TRUE
)

Arguments

srt

A Seurat object.

path

Path to the output .h5ad file.

features

Optional vector of features to include in the anndata object. Default is all features in assay_x.

assay_x

Assay to convert as the main data matrix in the anndata object. Default is "RNA".

layer_x

Layer name for assay_x in the Seurat object. Default is "counts".

assay_y

Assays to convert as layers in the anndata object. Default is c("spliced", "unspliced").

layer_y

Layer names for the assay_y in the Seurat object. Default is "counts".

reductions

Character vector specifying which Seurat reductions to convert into obsm. Default is NULL, which converts all available reductions.

graphs

Character vector specifying which Seurat graphs to convert into obsp. Default is NULL, which converts all available graphs.

neighbors

Character vector specifying which Seurat neighbor objects to convert into obsp. Default is NULL, which converts all available neighbor objects.

convert_tools

Whether to convert the tool-specific data. Default is FALSE.

convert_misc

Whether to convert the miscellaneous data. Default is FALSE.

overwrite

Whether to overwrite an existing file. Default is FALSE.

verbose

Whether to print the message. Default is TRUE.

Value

Invisibly returns the normalized path of the written file.

See also

srt_to_adata, h5ad_to_srt

Examples

if (FALSE) { # \dontrun{
data(pancreas_sub)
srt_to_h5ad(pancreas_sub, "pancreas_sub.h5ad")
} # }