Run doublet-calling with Scrublet — db_Scrublet • scop

This function performs doublet-calling using the scrublet(python) package on a Seurat object.

Usage

db_Scrublet(srt, assay = "RNA", db_rate = ncol(srt)/1000 * 0.01, ...)

Arguments

srt: A Seurat object.
assay: The name of the assay to be used for doublet-calling. Default is "RNA".
db_rate: The expected doublet rate. Default is calculated as ncol(srt) / 1000 * 0.01.
...: Additional arguments to be passed to scrublet.Scrublet.

Examples

if (FALSE) { # \dontrun{
data(pancreas_sub)
pancreas_sub <- standard_scop(pancreas_sub)
pancreas_sub <- db_Scrublet(pancreas_sub)
CellDimPlot(
  pancreas_sub,
  reduction = "umap",
  group.by = "db.Scrublet_class"
)

FeatureDimPlot(
  pancreas_sub,
  reduction = "umap",
  features = "db.Scrublet_score"
)
} # }