Generate proportion test plots based on the results from RunProportionTest.
Usage
ProportionTestPlot(
srt,
comparison = NULL,
FDR_threshold = 0.05,
log2FD_threshold = log2(1.5),
order_by = c("value", "name"),
pt.size = 1,
pt.alpha = 1,
cols.sig = "red",
cols.ns = "grey",
aspect.ratio = NULL,
xlab = "Cell Type",
ylab = "log2 (FD)",
theme_use = "theme_scop",
theme_args = list(),
legend.position = "bottom",
legend.direction = "vertical",
legend.title = "Significance",
combine = TRUE,
nrow = NULL,
ncol = NULL,
byrow = TRUE
)Arguments
- srt
A Seurat object containing proportion test results.
- comparison
A character string specifying which comparison to plot. If NULL, plots all comparisons.
- FDR_threshold
FDR value cutoff for significance.
- log2FD_threshold
Absolute value of log2FD cutoff for significance.
- order_by
Method to order clusters. Options: "name" (alphabetical), "value" (by log2FD value).
- pt.size
The size of the points.
- pt.alpha
The transparency of the points.
- cols.sig
Color for significant points and confidence intervals.
- cols.ns
Color for non-significant points and confidence intervals.
- aspect.ratio
The aspect ratio of the plot.
- xlab
A character string specifying the x-axis label.
- ylab
A character string specifying the y-axis label.
- theme_use
A character string specifying the theme to use for the plot.
- theme_args
A list of theme arguments to pass to the
theme_usefunction.- legend.position
Position of the legend.
- legend.direction
Direction of the legend.
- legend.title
Title of the legend.
- combine
Whether to combine the plots for each comparison into a single plot.
- nrow
An integer value specifying the number of rows in the combined plot.
- ncol
An integer value specifying the number of columns in the combined plot.
- byrow
Whether to arrange the plots by row in the combined plot.
Examples
data(pancreas_sub)
pancreas_sub <- RunProportionTest(
pancreas_sub,
group.by = "CellType",
split.by = "Phase"
)
#> ℹ [2025-11-13 12:01:42] Start proportion test
#> ℹ [2025-11-13 12:01:42] Running comparison: "G1" vs "S"
#> ℹ [2025-11-13 12:01:49] Running comparison: "S" vs "G1"
#> ℹ [2025-11-13 12:01:56] Running comparison: "G1" vs "G2M"
#> ℹ [2025-11-13 12:02:03] Running comparison: "G2M" vs "G1"
#> ℹ [2025-11-13 12:02:09] Running comparison: "S" vs "G1"
#> ℹ [2025-11-13 12:02:16] Running comparison: "G1" vs "S"
#> ℹ [2025-11-13 12:02:23] Running comparison: "S" vs "G2M"
#> ℹ [2025-11-13 12:02:30] Running comparison: "G2M" vs "S"
#> ℹ [2025-11-13 12:02:36] Running comparison: "G2M" vs "G1"
#> ℹ [2025-11-13 12:02:43] Running comparison: "G1" vs "G2M"
#> ℹ [2025-11-13 12:02:50] Running comparison: "G2M" vs "S"
#> ℹ [2025-11-13 12:02:56] Running comparison: "S" vs "G2M"
#> ✔ [2025-11-13 12:03:02] Proportion test completed
ProportionTestPlot(pancreas_sub)
# Plot specific comparisons
ProportionTestPlot(
pancreas_sub,
comparison = c("G2M_vs_G1", "G2M_vs_S")
)
# Plot paired comparisons using list format
ProportionTestPlot(
pancreas_sub,
comparison = list(c("G2M", "G1"))
)
ProportionTestPlot(
pancreas_sub,
cols.sig = "blue",
comparison = list(c("G2M", "G1"))
)